Siglec15 is a DAP12-related immune receptor, belonging to the immunoglobulin superfamily and SIGLEC (sialic acid-binding insulin-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They mainly exist on the surface of immune cells and are a subset of type I lectins. Siglec15 consists of an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail. Siglec15 recognizes sialic acid glycans and regulates osteoclast differentiation. Siglec15 is a potential therapeutic target for osteoporosis and has a conservative regulatory role in the vertebrate immune system. This product is Siglec15-FC recombinant protein (FC tag can be removed by enterokinase), which can be used for monoclonal antibody screening or antibody function verification.

Product name: Recombinant human siglec15 protein

Species: Human origin

Gene sequence number: Q6ZMC9

Tags: Human-Fc Tag

Tag site: C terminal

Restriction site: enterokinase

Expression area: extracellular segment 20-263AA

Host cell:CHO

Purity: ＞90%

Purification method: Protein A column

Preservation system: PBS+20% glycerol

Storage conditions: -80℃

Diagram: Lane M on the left is the protein MW marker, Lane 1 is the 15ug Siglec15-Fc protein;

The picture on the right shows the FACS detection of the  binding of Siglec15-Fc protein to 293T overexpressing  Siglec15- CAR

Reference materials:

1. Sialic acid binding Ig-like lectin 15 (SIGLEC15)

2. Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy