Mesothelin ( MSLN ) is a cell surface glycoprotein with a molecular weight of 40kDa . Mesothelin gene encodes a 69kDa premise protein, is formed by machining after a 40kDa membrane bound protein (of Mesothelin) and 31kDa called a megakaryocyte promoting factor (MPF) shedding of the extracellular fragment was released. The biological function of mesothelin is not yet clear, but due to its limited distribution in normal tissues, it is highly expressed in some tumor tissues. Therefore, it may be used for targeted therapy of tumors. There is evidence that mesothelin is An immunogenic protein, so it can be used to make anti-tumor vaccines. At present, a number of clinical trials of CAR-T targeting Mesothelin have also been carried out in China . This product is a MSLN- FC recombinant protein ( FC tag can be removed by enterokinase), which can be used for monoclonal antibody screening or antibody function verification.

Product name: Recombinant human MSLN protein

Species: Human origin

Gene sequence number: Q13421

Tags: Human-Fc Tag

Tag site : N- terminal

Restriction site: enterokinase

Expression area: extracellular segment ( 37-606AA )

Host cell : CHO-S

Purity: ＞ 90%

Purification method: Protein A column

Preservation system: PBS+20% glycerol

Storage conditions: -80℃

Illustration: Lane M on the left is protein MW marker, Lane 1 is 10ug MSLN-Fc protein;

The right image shows the FACS detection of MSLN protein binding to 293T overexpressing anti-MSLN-CAR.

Reference materials:

1. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers

2. Mesothelin-family proteins and diagnosis of mesothelioma

3. Mesothelin: A new target for immunotherapy