BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein that recognizes the TNF receptor superfamily of BAFF. B cell activating factor (BAFF) can improve the survival rate of B cells in vitro and is a regulator of the peripheral B cell population. Overexpression of BAFF in mice leads to the proliferation of mature B cells and symptoms of systemic lupus erythematosus (SLE). In addition, some patients with SLE have elevated serum BAFF levels. Therefore, it has been proposed that abnormally high levels of BAFF can promote the onset of autoimmune diseases by enhancing the survival of autoreactive B cells. This product is a second-generation CAR lentiviral expression plasmid targeting BAFFR.

Product name: Lenti-EF1a-BAFFR-2nd-CAR-EGFRt

Promoter: EF1a ^[1]

Intracellular co-activation signal: 4-1BB/CD3Zata

Resistance gene: Amp

Tag: EGFRt ^[2]

Remarks:

1. Compared with the CMV promoter, EF1a can be continuously expressed in immune cells, so it can significantly extend the expression of CAR in T cells.

2.  EGFRt intercepts domain III and domain IV of wild-type EGFR. This truncated EGFRt has no intracellular signal and will not transmit other signals to T cells . At the same time, these two domains are cetuximab Recognizing the epitope and expressing the epitope on T cells can not only serve as a CAR-T screening marker, but also add a safety switch for clinical research. Related flow cytometry antibodies can be inquired ( iCareAb, CAT# IAB006A)