HER2 receptor (also called c-erbB-2, HER2/neu) is a transmembrane protein with a molecular weight of 185kD and tyrosine kinase activity. Its coding gene is located on human chromosome 17q21 and is a member of the epidermal growth factor receptor family . HER2 receptor (also called c-erbB-2, HER2/neu). Her2 is composed of three parts: extracellular domain (ECD), transmembrane domain and intracellular domain (ICD). The extracellular domain is mainly composed of two ligand binding domains (RLD) and two cysteine-rich domains. The acid region constitutes. The HER2 monomer is inactive and must form a dimer to generate an activation signal. The HER2 heterodimer greatly weakens the coupling between EGFR and cab1, reduces the degradation of EGFR in the process of endocytosis, and promotes EGFR Circulates back to the cell membrane, causing EGFR to be overexpressed in the cell membrane, accelerating cell proliferation and accelerating tumor formation and growth. Since the overexpression of Her2 is closely related to tumor occurrence and development, it is an ideal target for tumor targeted therapy. This product is a second-generation CAR lentiviral expression vector targeting Her2, which can be used for in vivo and in vitro verification.

Product name: Lenti-EF1a-Her2-2nd-CAR

Promoter: EF1a ^[1]

Intracellular co-activation signal: 4- 1 BB /CD3Zata

Resistance gene: Amp

Tag: EGFRt ^[2]

Remarks:

1. Compared with the CMV promoter, EF1a can be continuously expressed in immune cells, so it can significantly extend the expression of CAR in T cells.

2. EGFRt intercepts domain III and domain IV of wild-type EGFR. This truncated EGFRt has no intracellular signal and will not transmit other signals to T cells. At the same time, these two domains are cetuximab Recognizing the epitope and expressing the epitope on T cells can not only be used as CAR-T screening markers, but also add a safety switch for clinical research. Related flow cytometry antibodies can be inquired ( iCareAb, CAT# IAB006A )